Media preparation, bacterial cell culture and identification, prokaryote cell number, isolation of genomic DNA from bacteria, restriction enzyme digestion of DNA, ligation of plasmid DNA to insert DNA, transformation of E. coli by electroporation, polymerase chain reaction, agarose gel electrophoresis, total protein isolation, poly acrylamide gel electroporation

Pre-requisites: BIOE320*

Co-requisites: BIOE 320